The Total Thyroxine (T4) ELISA is a solid-phase enzyme-linked immunosorbent assay based on the principle of the competitive ELISA technique for quantitative determination of Thyroxine in human serum.
The three main kit components are:
1) Solid microwells pre-coated with anti-T4
2) T4 Calibrators
3) Liquid T4 Enzyme Conjugate Mixture comprised of T4 labelled with horseradish peroxidase (T4-HRP)
Product Freature:
Analytical Sensitivity: 176 aµg/dL at 2-SD
Specificity: No cross-reactivity or interference with
d-Thyroxine 10 aµg/mL
d-Triiodothyronine 100 aµg/mL
Iodotyrosine 100 aµg/mL
Diiodotyrosine 100 aµg/mL
TBG 50 aµg/mL
Human Albumin 50 aµg/mL
Phenylbutazone 25 aµg/mL
Phenytoin 50 aµg/mL
ASA 500 aµg/mL
Acetaminophen 500 aµg/mL
Accuracy: correlation coefficient 0.98 with a reference method
Intra Assay Precision: CV a?¤ 5.9%
Inter Assay Precision: CV a?¤ 7.6%
Product Specification :
Anti-T4 Ab Coated Microwells
Total T4 Calibrators:
C1 (0 ng/mL)
C2 (2.0 ng/mL)
C3 (5.0 ng/mL)
C4 (10.0 ng/mL)
C5 (15.0 ng/mL)
C6 (25.0 ng/mL)
Total T4 Assay Control
Total T4 Enzyme Concentrate
Enzyme Buffer
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Product Insert
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