Rapid Antigen and Antibody test kit covid 19
Manufacturer: Shenzhen Lvshiyuan Biotechnology Co., Ltd / Green Spring Brand
The time required: 15 -20 minutes
Detect the virus 3 days after the infection
Accuracy: High
Specificity: High
Cost Saving : Low cost
Intended use:
AIVD COVID-19(SARS-CoV-2) IgG/IgM Rapid Test is a single use, rapid device for qualitative detection of total antibodies against 2019 novel coronavirus (SARS-CoV-2) in human serum, plasma or whole blood specimens. The kit is intended for screening of patients suspected for infection with SARS-CoV-2, and as an aid in the diagnosis of the coronavirus disease 2019 (COVID-19).
Descriptionï¼?
Novel coronavirus pneumonia (NCP) or SARS-COV-2, that was officially named by the WHO as â??Corona virus disease 2019â?? (COVID-19), is a respiratory infection caused by a new virus that was first identified in late 2019.
IgM/IgG Rapid Kit can specifically detect IgM and IgG antibodies of COVID-19, covering the entire course of infection and recovery period of patients. This approach improves the overall diagnosis of COVID-19 in both acute and recovery phases of illness.
This kit utilizes the principle of colloidal gold immunochromatography to qualitatively detect the new coronavirus (COVID-19) IgG / IgM antibodies in human serum and plasma. Lateral flow immunochromatographic assay provides an easy workflow, short turnaround time, and rapid diagnosis of COVID-19 suspected patients.
This method does not require expensive medical instruments and consumables. Just take the specimen and add it to the sample well, and then add the required amount of a diluent. The IgG / IgM antibodies in the sample will interact with the colloidal gold-labeled COVID-19 recombinant antigen on the conjugate pad. Then the conjugate pad will release re-solubilized conjugate onto the nitrocellulose membrane. The nitrocellulose membrane (NC membrane) diffuses it forward.
As the sample moves along the device binding reagents situated on the nitrocellulose membrane bind to the target at the test line. If the sample contains a COVID-19 IgG antibodies, they will bind to the colloidal gold-labeled novel coronavirus recombinant antigen, diffuse forward, and then react with the anti-human IgG antibodies immobilized on the NC membrane detection line (T2 line). Similarly, if the specimen contains IgM antibodies to COVID-19, the antibodies will react with antigen-coated particles, the conjugate migrate laterally forward, and cause a colored line (T1 line). The darker the color of colloidal gold on the test line, the higher the concentration antibodies to COVID-19 in the sample.
Accuracy:ï¼?> 90%
Specificity: 97%
Basic Protocolï¼?
1. Remove the test specimen, required reagents from storage conditions, and equilibrate to room temperature.
2. Unpack the aluminum foil bag, place the test horizontally on the table and number it.
3. Add 10ul serum, plasma or whole blood sample to the sample well with a pipette or a dropper. Slowly add 2 drops of sample dilution (about 60uL) to the sample well.
4. Read the results within 10-15 minutes after adding the sample, and the results will be invalid after 30 minutes.
This kit utilizes fluorescence quantitative probe-based PCR and guarantees a high specificity to ensure accurate one-step identification of ORF1ab and N genes of 2019-nCoV. The kit offers a highly sensitive test with a limit of detection as low as 500 copies/ml. The results are available within 1.5 hour.
Kit Contents (48 Tests /Kit)
Dual Enzyme solution A 50 µl
Dual Ncvo-O / N reaction solution A 1 ml
Dual Ncvo-O / N positive control A 200 µl
Dual Negative control A 200µl
Applicable equipment
ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments.
Specimens requirements
Specimen type: nasopharyngeal swab from suspected infection; virus cell culture fluid, etc.
Basic Protocol
1. Sample preparation.
Take the test sample and extract the RNA nucleic acid according to the instructions of the nucleic acid extraction kit. The nucleic acid extraction product should be stored at -20 C.
2. Reaction mixture preparation
According to the total number of test samples, the number of PCR reaction tubes needed is. N = number of samples + 1 negative control + 1 positive control.
The following protocol is recommended for a 20 µl reaction volume. If the volume of reaction changes, please adjust proportionally.
Components Volume
Dual Enzyme solution A 1 µl
Dual Ncvo-O / N reaction solution A 19µl
Final Volume: 20 µl
3. Sample adding.
Add 5 µl each of the extracted RNA, positive and of negative control to corresponding reaction tubes. After assembling all the components, cover the tube caps and gently mix the contents of the tube, mix well, and centrifuge briefly.
4. Perform quantitative PCR
Place the reaction tube inside a real-time PCR instrument.
Set the channel and sample information, reaction system volume 25 µl.
Select the following channels: FAM channel for nCOV-ORF1ab, VIC channel for nCOV-N.
Perform quantitative PCR using recommended cycling parameters settings:
Step Temperature Time Number of cycles Fluorescence Detector
1 50C 15 min 1 Off
2 95C 3 min 1 Off
3 95C 10sec 45 Off
60C 30 sec On
Result analysis:
1) Set the baseline: Generally, it is set to 6-15cycle for ABI 7500, 7700 and other instruments, 3-15cycle for PE5700, and 6-12cycle for MJ Research Option2. Under special circumstances, the baseline can be adjusted accordantly.
2) Set the threshold: The threshold line just exceeds the highest point of the negative control amplification curve (random noise line).
AIVD Biotechs COVID-19 Ag test is a lateral flow immunoassay intended for the qualitative detection of nucleocapsid protein (NP) antigen from SARS-CoV-2 in direct nasal swabs from individuals suspected of COVID-19.
Description
This kit utilizes the principle of latex bead immunochromatography to qualitatively detect the new coronavirus (COVID-19) Np antigen in human nasal swabs. Lateral flow immunochromatographic assay provides an easy workflow, short turnaround time, and rapid diagnosis of COVID-19 suspected patients.
The test contains latex bead conjugate pad and a membrane strip pre-coated with antibodies specific to SARS-CoV-2 antigen on the test lines (T). If SARS-CoV-2 antigen is present in the specimen, a visible band appears on the test lines (T) as antibody-antigen-antibody latex bead complex forms. The control line (C) is used for procedural control and should always appear if the test is performed correctly.
This method does not require expensive medical instruments and consumables. Just take the specimen and add it to the sample well. If sample contains the antigen, it will interact with the latex bead-labeled COVID-19 anti-SARS-COV-2 NP antibodies on the conjugate pad.
Required components
1. A foil pouch with a desiccant and a single-use test card, 1 piece
3. Sterile swab, 1 piece
3. Extraction buffer tube (1 ml), 1 tube.
4. Instructions for use, 1 piece.
Specimen requirements:
1. Nasopharyngeal swab specimen.
2.Specimen should be tested as soon as possible upon collection. If the sample has to be stored, store the swab sample at room temperature for up to 1 hour or 2~8â?? for up to 4 hours prior to testing.
Specimen preparation
1. Insert the extraction buffer tube into the tube rack, make sure that the sample tube is firmly upright and touches the bottom of the rack.
2. Insert the swab into an extraction buffer tube.
3. Gently stir the swab in the tube, and then left swab inside the tube for 1 minute or Mix on the vortex mixer for about 30 seconds,
4. The sample should be tested immediately after collection.
If it cannot be tested in time, it should be stored in the virus transport matrix. The specimens can be stored at 2-8C for 4 hours
Basic Protocol
Step 1: If the sample is refrigerated, remove the sample to be tested and the required reagents from the storage conditions, equilibrate to room temperature (15~30C)
Step 2: When preparing to test, open the aluminum foil bag, take out the test card, and lay it flat on a table.
Step 3: Mark the sample number on the test card,
Step 4: Using pipette add 50ul of the sample into a sample well on the device,
Step 5: Read the result within 15 minutes,
Please read the result after 15 minutes. After observing and recording the result, please discard the test card to avoid affecting the result judgment.
Do not read test results after 30 minutes. It may give false results.
Made of genuine leather.
Tapered round u-shaped key holder.
Delicately trimmed and stitched for added exquisiteness.
Allow you to pack up and take out keys easily.
Sturdy and durable construction.
Size: 12 x 7.2 x 1cm.
Patent product.
With minimalist detailing and an embossed logo on the front.
Ideal to be used as company promotion.
Suitable for men or women.
Keep keys neatly organized in this refined leather bag.
A stylish yet versatile leather key holder that is never go out of style.
1. Material:acrylic+resin, no toxic, safe, tasteless.
2. Unique attractive appearance, set for the integration of utility and ornamental, rich color water and floating objects.
3. The first choice for gifts or promotional advertising.
4. Can change anything based on customer requirements, can set or print advertisement on colored water or floating objects.
With this software you can program keys/transponders for vehicles, bikes or trucks from different brands that using Megamos 13, Megamos 48 (Megacrypt I), Megamos 8E (Megacrypt II), Texas 4C, Temic 11, Temic 12, T5, TK5555, Philips PIT - 7930/7931/7935 (PIT), Philips HITAG2 ¡§C 7936, 7941, 7942, 7943, 7944, 7945, 7946, 7947, 7952, 7953, 7961
Transponder programmers. Just need dump from your immobilizer, put your NEW KEY (or transponder) on the programmer and generate working key! You can also produce preprogrammed transponders for a different vehicles.
This programmer works with AVDI interface. It also is compatible with our software ABRITES Commander for VAG, BMW, Renault, Nissan, Mercedes, Fiat.
If you lost the key programmer, you can order our device ,but you must have the authorised software.
The professional duplicating machine, AD900 Pro, has been designed and built to keep pace with the evolution of transponder car keys.
The AD900 Pro key duplicator features the most innovative electronic components in the field of radio frequencies thus allowing easy detection, reading and cloning (duplication) of fixed code transponders and the identification of cryptographic transponder codes.
Major features00Pro such include the ability for copying of Crypto 42 type transponders and the Texas 4C and 4D transponders.
The AD900 Pro has been developed to cater for future developments and to keep up to date with the ever expanding technology of transponders within the Automotive vehicle systems.
The AD900 Pro offers complete flexibility as once the main unit is purchased the functionality can be expanded by adding additional software.
The machine of the future for transponder developments, is the AD900 Pro for professional locksmiths.
USB2.0 interface
Available in Colors
Storage capacity: 512Mb to 16GB flash memory
High speed data transfer performance
Hot plug and play
Support multiple operation systems: Windows, Macintosh, Linux
10 years data retention More than 1,000,000 times of usage
Certificate:CE,FCC and ROHS
Packing:polybag, Plastic box, blister, gift box or customized
Delivery: DHL,TNT,UPS,Fedex,by Air
Dummy samples available
OEM orders are welcome
SBB Key Programmer(Version V33) adds Ford 2010 and Hummer.
V33 is the latest version now ! older is v32 ,v31 and v28
It is known that China sbb key programmer is the best key maker for European car, compared to T300 key programmer and ad900. Eobd2 Eshopí»s SBB key programmer now updated to the newest version V33. If you are looking for a best key programmer for Ford 2010 or Hummer, you will find Sbb key pro v33 can make it, during sbb key programmer carlist, it adds Ford 2010 and Hummer.Here"s the picture of SBB Key Programmer V33.
SBB Key Programmer V33 add 2010 Ford and Hummer
SBB is a universal key programmer for it can program multi-brands car keys, because its software with full database containing the most important vehicle makes. Compare to the former version which support global cars, SBB key programmer V33 support new cars likes Ford 2010 and Hummer.
SBB V33 Key maker function
1. Programming new keys
2. Reading keys from immobilizer"s memory /pin code
3. Reactivating keys no longer in the car owner"s possession
4. Operation by means of a menu guided programming
5. Programming new remote controls.
How SBB universal key maker Works :
SBB key programming tool can still make key even when the customers lost their automobile keys, because SBB interface is to insert into the OBD diagnostic seat to read chip data.
The steps of SBB make car keys:
1) Before running SBB key programmer, please insert the blank new key into your car keyhole;
2) Turn on ignition switch;
3) Insert SBB 16-pin interface into your car OBD2 diagnostic seat. SBB key programmer copies chip according to car model, and it program key not clone key, it only needs to insert SBB 16-pin interface into your car OBD2 diagnostic seat, then read out immobilizer pin code from immobilizer dump/eeprom of the car before writing it into new blank key. When programming Ford vehicle, it does not need pin code.
SBB key programmer is better for European vehicles, and it is no token system.
SBB copies chip according to car model, while AD900 does according to chip model. SBB key programming tool can still make key even if all of keys lost, because SBB interface is to insert into the OBD diagnostic seat to read chip data; AD900 can write the chip of an old key into a new key or empty key. And SBB can read immobilizer pin code as MVP and T300 do, while AD900 can not read immobilizer pin code, AD900 can not read immobilizer pin code, AD900 can directly copy and duplicate keys.
Simple, powerful, essential...Advanced key coding made easy!
The world's most powerful key programmer
The ad100 pro has been approved by the ala (auto locksmith association cn) and is used extensively by most of their members.
It connects easily to the vehicles serial diagnostic port and the built in
Instructions make the system simple to use, powerful, and a tool ideally suited
For vehicle locksmiths and security professionals alike.
With an extensive software applications portfolio and constant development the
Ad100 pro is a must! it now opens new opportunities for anyone involved in
Vehicle immobiliser systems, transponders, key cutting & programming.
The ad100 pro diagnostic system is essential for dealing with the latest vehicle
Technology and immobiliser systems - there is no better...
Ad100 pro key programming
Features:
Programs new keys
Ecu identification
Reads eka codes
Programs new eka codes
Programs plip keys
Reads & erases fault codes
Displays live data
Component activation
Reads immobilisers ecu id's
Reads mechanical key codes
Clears key memories
Kit includes:
1kit ad100 pro hand held tester
2pc master obd cables
1pc usb cables
1book operating manual
1book tips & hints updates
1dvd software
7pc dongle
1box protective carrying case
Kit includes:
1kit ad100 pro hand held tester
2pc master obd cables
1pc usb cables
1book operating manual
1book tips & hints updates
1dvd software
7pc dongle
1box protective carrying case
Mvp means multi vehicle programmer, mvp is made by advanced diagnostics limited, it is the update version of perfect product ad100.
The idea of mvp is: you only pay a little money and you can use the original full functions code read by installment plan. You can pay token when you make the key.
Covers all version functions of t300, automam, k1, 2005, 2008, decoder), and add more functions, such as the update 06-07 honda system, peugeot system and control, hyundai system and control, the menu and control of daewoo, kia control, gm control and system update, mitsubishi can bus system, vw menu and control, can bus interface.
The function:
1. Read the default code of car
2. Cleanup all default code
3. Read the data of system
4. Read the immo/ecu id code
5. Read the code of mechanical key
6. Cleanup the memory of car key
7. Programmer a new key
8. Read the enter code of car key
9. Discern the type of ecu
10. Programmer ecu-immobiliser
11. Read the eka code
12. Programmer the new eka code
13. Programmer remote device
14. Matching the intelligent key
15. Matching the intelligent remote key
Added the model after update:
1. Saab-matching key of can bus system
2. Honda(07 civic)
3. Toyota/lexus-can bus system
4. Volvo-key matching
5. Mitsubishi-can bus system
6. Hyundai/kia-can bus system
7. Nissan-matching intelligent remote key
8. Vag read code function
9. Rover-mg and so on.
Kits includes
1. Mvp hand held tester x 1pc
2. 9 inch main cable x 1pc
3. Car connector x 12pcs
4. 12v power x 1pc .
