Bovine Tuberculosis Ab Test

4. Centrifugal tubes (each containing 180 ¦ìl of assay buffer)
5. Product manual


Test procedure

1. Do centrifugation to cattle¡¯s whole blood sample to collect serum or plasma for use.
2. Use small pipette with reticle to transfer 20 ¦ìl (upper reticle) of serum or plasma into the centrifugal tube with assay buffer. Mix them well by the pipette attached in the pouch.
3. Take out the cassette from the foil pouch and place it horizontally.
4. Drip 3 drops (about 100 ¦ìl) of sample mixture into the sample hole.
5. Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

Interpretation of results

Positive: the presence of both c band and t band, no matter t band is clear or vague.
Negative: only clear c band appears.
Invalid: no colored band appears in c zone, no matter whether t band appears.



Storage

The kit can be stored at room temperature (2-30¡ãc). The test kit is stable through the expiration date (18 months) marked on the foil pouch. Do not freeze. Do not store the test kit in direct sunlight.


Precaution

1. For best results, please strictly adhere to these instructions.
2. All reagents must be at room temperature before running the assay.
3. Do not remove test cassette from its pouch until immediately before use.
4. Do not reuse the test kit.
5. Do not use the test beyond its expiration date marked on the foil pouch.
6. The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

Limitation

Bovine tuberculosis ab rapid test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as elisa for final determination in practice.




In house standard