In laboratories, the most common experimental objects are cells. The safety of cells is related to the accuracy of experimental results. Therefore, they need to be stored in a low-temperature environment. At this time, experimenters usually choose a cell freezing container, using -196�° liquid nitrogen to maintain low temperature. Its vacuum structure can provide a stable low-temperature environment for cells. However, there are also disadvantages. Liquid nitrogen is easily volatile. Although the vacuum structure can effectively reduce it, the surrounding environment will also have an impact.
Therefore, when using cell freezing containers, there are requirements for the storage environment.
Ambient temperature requirements:
A suitable temperature will reduce the volatilization of liquid nitrogen, while an excessively high temperature will accelerate the volatilization.
Just like summer and winter, the temperature difference between the two seasons is large, and the volatilization in summer will be much higher than that in winter.
The suitable temperature for cell freezing containers is about 20�°, and they can be stored in a cool room or dark place, away from direct sunlight and high temperature environments, to reduce the impact of heat on the tank.
Ventilation requirements:
It should be placed in a well-ventilated area, away from sources of fire and flammable materials. In order to prevent the discharge of volatile liquid nitrogen, the environment is too sealed and cannot dissipate quickly. It also competes with oxygen for space, causing workers to suffer from hypoxia and suffocation.
Storage requirements:
The inner and outer bladders of the container are only connected by the neck tube, which is relatively fragile. To prevent the neck tube from rupturing due to stress, we should store it vertically. At the same time, pay attention to avoid collisions during the movement of the jar to prevent the neck tube connection from falling off and causing damage to the vacuum.
This is the storage environment requirement of cell freezing containers.
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