Tromethamine Buffer Of Desheng

The Chinese name of Tris is tris(hydroxymethyl)aminomethane, tromethamine, tromethamine, 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.
Tris has a high buffer capacity, high solubility in water, and is inert to many enzymatic reactions, making Tris a very satisfactory buffer for many biochemical purposes. It is generally used to stabilize the pH of the reaction system, and has a strong buffering capacity between pH 7.5-9.0.
Advantages of Tris buffer:
1. Because Tris base is more alkaline, you can only use this buffer system to configure a buffer with a wide range of pH ranging from acidic to alkaline;
2. It has little interference to biochemical processes, and does not precipitate with calcium, magnesium ions and heavy metal ions.
Disadvantages of Tris buffer:
1. The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1;
2. The temperature effect is large, and the temperature change has a great influence on the pH of the buffer. The pH of the buffer at 4�?�°C is 8.4, and the pH at 37�?�°C is 7.4. It must be prepared at the temperature of use, and prepared at room temperature Tris-HCL buffer cannot be used for 0-4;
3. It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
4. This buffer has a certain interference effect on some PH electrodes, so use electrodes that are compatible with Tris solution.
Application of Tris buffer:
It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH; uses the Tris-HCL buffer system in the gel to stabilize the pH; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of the Tris buffer can also be used The formation of intermediate fibers of nematodes; add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; change the pH-adjusting acid solution to acetic acid to obtain "TAE buffer", change "TBE buffer" can be obtained as boric acid. These two solutions are used in nucleic acid electrophoresis experiments.

Every biological system has a well-known buffer system to maintain the acid-base balance within a specific pH range. Our lives depend on the function of the buffer system. A buffer system is a solution that resists changes in pH when acid or alkali is added. Different buffers have different ability and efficacy for skin replacement. The high-quality Good's Buffers produced by Desheng are usually used in cosmetics, such as toners, essences, lotions, eye creams and facial masks, etc., according to your application. Here are 4 biological buffers commonly used in cosmetics.
1) HEPES (4-hydroxyethylpiperazine ethanesulfonic acid)
CAS Number: 7365-45-9
Useful pH range: 6.8-8.2
The main purpose
1. Acidity regulator, or pH control agent.
2. An effective concentration of HEPES can provide an exfoliating effect.
3. Promote the transdermal absorption of various functional ingredients in cosmetics.
2) TRIS (Tris(hydroxymethylaminomethane))
CAS number: 77-86-1
Useful pH range: 7.2-9.0
use:
1. Non-ionic surfactants-amines
2. Acidity regulator or pH control agent.
3. Excellent cleaning ability and emulsifying ability.
3) BIS-TRIS (Trimethylol) Methylamino Propane)
CAS Number: 5625-37-6
Useful pH range: 6.1-7.5
The main purpose
1. Non-ionic surfactants-amines
2. Acidity regulator, or pH control agent.
3. Excellent decontamination, dispersion, emulsification and antistatic ability.
4) Glycylglycine buffer (G-132)
CAS Number: 7365-44-8
Useful pH range: 7.5-8.9
The main purpose:
1. Adjust the pH range of the solution
2. Condition the skin, reduce pores and prevent skin aging.
3. Excellent emulsification, dispersion, antistatic, wetting, penetration, and decontamination capabilities
Precautions
External factors, water and detergents may reduce the local buffering capacity, because the elution of buffer chemicals leads to increased pH and irritant contact dermatitis. Inflammatory diseases, including atopic dermatitis, psoriasis, and acne vulgaris, increase in pH and decrease buffering capacity. For the treatment of skin diseases and aging skin, emollients with a pH value slightly higher than the average normal pH value and with appropriate buffering capacity should be used first.
Desheng is a professional manufacturer of biological buffers. There are many cooperative manufacturers in the skin care industry. In addition to the two biological buffers Hepes and Tris, it also produces many other models such as Mops, Taps, Pipes, Caps, and the core product tris buffer. Known for its ability to maintain the original color of emulsions and excellent odor suppression capabilities, it is widely used in cosmetics such as skin care lotions, sunscreens, eye makeup products, nail polish, etc. It can maintain the functional effectiveness of active substances and is a skin care product The industry's trusted choice.

1. Tris brief introduction
As the handling in biological buffers, tris plays a more important role, not only because of the wide pH range of tris, it can act as a pH regulator. Conventionally, it is white crystal particles, but there are certain requirements for water solubility, and white powder will not meet its requirements better. The pH value of the acid-base indicator is 7-9, and there are other names, tromethamine, ammonium tromethamine, trishydroxymethylaminomethane and so on. Although they are all the same product, different buyers will call them differently based on different fields.
Second, the use of Tris
2. Tris application in cosmetics
With the improvement of peoples quality of life, more and more attention is paid to spiritual life. Among them, the heart of beauty is common to everyone, regardless of men and women: appearance has become an important indicator of first impression (of course I strongly disagree Because I dont have that beauty. The rise and mass use of cosmetics have become inevitable. To make good cosmetics, the ingredients are very important.
The role of Tris in cosmetics is basically to adjust the pH, and it can also be used as a neutralizer. The PH value of healthy Asian skin is slightly acidic, and the ingredients in many cosmetics are very complicated. If you want to adjust the pH value, you must use tris to adjust, so that the entire raw material composition will not change too much. Many people see that tromethamine is a chemical product, and it is very scary to use in cosmetics, and they are afraid of being unsafe. In fact, if the proportion of ingredients is used properly, there is no need to worry about it. Generally, it will not harm the skin.
3.Tris is used for synthesis
Tris with a relatively simple structure can synthesize many products, and the same buffer system, Tris-HCL, Bis-Tris Tricine, TES, TAPS, etc. can all be synthesized with tris. Compared with others, the price of Tris will be cheaper, but the synthesized products can be applied to more fields, and the price will be doubled. Therefore, the synthesis of tris can be said to be a hot pastry.

4-Hydroxyethylpiperazine Ethane Sulfonic Acid (HEPES) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research.
As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products:
1. Advantages of PH range:
The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture.
Purity advantage:
Desheng HEPES has a purity of >=99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services.
Reserve advantages:
Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment.
Export advantages:
Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention.
HEPES packaging advantages:
Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.

In our daily life, sodium citrate appears in all kinds of raw materials around us. How many people have known about it? Are you clear about its functions, features and uses? Let's take a look at some of its secrets.

Sodium citrate is the most important citrate at present, which is mainly produced by fermentation of starch and neutralization with alkali

Desheng biotechnology is a professional manufacturer of vascular additives, in vitro diagnostic reagents, biological buffers, luminescent substrates and enzyme preparations. It has independent intellectual property rights and professional R & D and production capacity in the aspect of blood vessel additives, and can produce a variety of products. Desheng has been established for many years and has rich production experience. At present, its products are sold to more than 100 countries all over the world with excellent quality and favorable price. Interested in understanding can call consultation, Desheng welcome your call.

|English name|3-Morpholinopropane sulfonic acid

|Chinese name|3-Morpholinopropane sulfonic acid

|Abbreviation|MOPS

|CAS#|1132-61-2

|Molecular weight|209.26

|Molecular formula|C7H15NO4S

|Storage condition|room temperature, away from light and moisture

|Application|Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.

Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer.

What is PIPES buffer
PIPES Buffer (CAS5625-37-6) chemical name is 1,4-piperazine diethanesulfonic acid, which is a zwitterionic biological buffer. It is a white powder solid at room temperature. PIPES Buffer is not easily soluble in water (1g/L (100C). It can be dissolved by mixing equimolar sodium salt solution and titrating to the appropriate pH value. For example, PIPES Buffer (CAS76836-02-7) and PIPES sesquisodium salt ( CAS100037-69-2).
Application of PIPES Buffer
PIPES Buffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscopy research. PIPES Buffer is similar to MES Buffer and MOPS Buffer (also produced by Desheng). This Good's Buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non-coordinating buffer in metal ion solutions.
Advantages of PIPES Buffer
According to related literature, the use of NaOH-PIPES buffered glutaraldehyde solution in the ultrastructure of various tissues, such as human skin, human hypertrophic scars, mollusk neuron tissue, etc., the results show that more organelles and organelles can be observed Between the details. The cytoplasm is more uniform in consistency. Compared with NaOH-PIPES buffer, microfilaments and microtubules are more common in PIPES tissues. In addition, PIPES Buffer is proven to be comparable in living cells.
What is TRICINE buffer
TRICINE Buffer (CAS 5704-04-1), a zwitterionic biological buffer containing amine and acid groups. This product is a white crystalline powder, slightly soluble in water (the solubility is 1.8g/100ml water).
Application of TRICINE Buffer
TRICINE is widely used in cell culture and electrophoresis research. As a buffer for cell culture research, TRICINE can inhibit the unexpected growth of mycoplasma in animal tissue culture. It has also been used in bacterial culture to prevent precipitation of iron salts. The Good's buffer can form complexes with most metal ions, such as Mg, Ca, Co, Cu, Ni, and Zn, so the stability constant and concentration should be considered when choosing the buffer. As a buffer for electrophoresis research, Tricine has been used as an alternative to protocols using the controlled substance barbital.
Advantages of TRICINE Buffer
TRICINE has a higher negative (more negative) charge than glycine, making it migrate faster. In addition, its high ionic strength results in more ion movement and less protein movement, so that low-molecular-weight proteins can be separated in a lower percentage of acrylamide gels. Tricine has been proven to be used to separate proteins in the range of 1 to 100 kDa by electrophoresis. In ten tests using firefly luciferase for ATP determination, 25mmol/L tricine buffer was found to be the most effective buffer.

In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment.
When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers.
Prepare TRIS base and TRIS-HCL buffer
Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O.
Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L.
Prepare HEPES buffer
To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C.
Prepare PIPES buffer
To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O.
Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.

When many suppliers think of Carbomer 940, they first think of Lubrizol or Godsend. Because of the monopoly of Lubrizol's cooperation with the brand, many customers have lost their supply chains. Today, letâ??s take a look at other Carbomer manufacturers besides Lubrizol and Tianci.
When looking for suppliers of Carbomer 940, everyone is accustomed to searching on search engines, such as Baidu and 360. I donâ??t know if you have noticed. Deshengâ??s ranking is often relatively high. Deshengâ??s full name is Hubei Xindesheng. Material Technology Co., Ltd., established in 2005, specializes in the production and development of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. Carbomer is the top priority of the company's products. Desheng specially hires professional and technical personnel to continuously improve product quality, and has a dedicated quality department to conduct inspections. Unqualified products will never reach customers.

In order to capture the carbomerâ??s market enthusiasm, many manufacturers purchase carbomer raw materials at low prices and provide them to purchasers, resulting in many unqualified phenomena after use. Desheng is a professional carbomer manufacturer with good quality products. Moreover, it is cost-effective. Now we are launching year-end discounts. If the order is large, the price is as low as 100 yuan/KG. Under the premise of ensuring product quality, Desheng provides perfect after-sales service, and promises that if there is any quality problem, it will be free The return and exchange provide buyers with product quality assurance and solve customers' troubles.
Although there are many series of Carbomer, Desheng mainly produces Carbomer 940 and Carbomer 980, because these two kinds of applications are the most. It should be noted that carbomer has strong moisture absorption, so it must be sealed when storing, and beware of moisture absorption and agglomeration in the air. The carbomer produced by Desheng is packed in non-toxic polypropylene plastic bags. , Moisture-proof and air-tight, and then packed in cardboard drums. The conventional packaging is 10KG/bag. Desheng can pack according to customer needs. Samples can be provided, and business friends in need can directly contact the official website customer service to obtain the use kit.