Rt-Qpcr Detection Kit

Description

This kit utilizes fluorescence quantitative probe-based PCR and guarantees a high specificity to ensure accurate one-step identification of ORF1ab and N genes of 2019-nCoV. The kit offers a highly sensitive test with a limit of detection as low as 500 copies/ml. The results are available within 1.5 hour.

Kit Contents (48 Tests /Kit)
Dual Enzyme solution A 50 µl
Dual Ncvo-O / N reaction solution A 1 ml
Dual Ncvo-O / N positive control A 200 µl
Dual Negative control A 200µl

Applicable equipment
ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments.

Specimens requirements
Specimen type: nasopharyngeal swab from suspected infection; virus cell culture fluid, etc.

Basic Protocol
1. Sample preparation.
Take the test sample and extract the RNA nucleic acid according to the instructions of the nucleic acid extraction kit. The nucleic acid extraction product should be stored at -20 C.
2. Reaction mixture preparation
According to the total number of test samples, the number of PCR reaction tubes needed is. N = number of samples + 1 negative control + 1 positive control.
The following protocol is recommended for a 20 µl reaction volume. If the volume of reaction changes, please adjust proportionally.

Components Volume
Dual Enzyme solution A 1 µl
Dual Ncvo-O / N reaction solution A 19µl
Final Volume: 20 µl

3. Sample adding.
Add 5 µl each of the extracted RNA, positive and of negative control to corresponding reaction tubes. After assembling all the components, cover the tube caps and gently mix the contents of the tube, mix well, and centrifuge briefly.

4. Perform quantitative PCR
Place the reaction tube inside a real-time PCR instrument.
Set the channel and sample information, reaction system volume 25 µl.
Select the following channels: FAM channel for nCOV-ORF1ab, VIC channel for nCOV-N.
Perform quantitative PCR using recommended cycling parameters settings:

Step Temperature Time Number of cycles Fluorescence Detector
1 50C 15 min 1 Off
2 95C 3 min 1 Off
3 95C 10sec 45 Off
60C 30 sec On
Result analysis:
1) Set the baseline: Generally, it is set to 6-15cycle for ABI 7500, 7700 and other instruments, 3-15cycle for PE5700, and 6-12cycle for MJ Research Option2. Under special circumstances, the baseline can be adjusted accordantly.
2) Set the threshold: The threshold line just exceeds the highest point of the negative control amplification curve (random noise line).

ANDiS SARS-CoV-2 RT-qPCR Detection Kit
ANDiS SARS-CoV-2 and Influenza A/B RT-qPCR Detection Kit
ANDiS 350 Automated Nucleic Acid Extraction System

Temperature
Block Temperature Range: 0 - 105
Max. Heating Rate: 7 /s
Max. Cooling Rate: 5 /s

Application
Suspected case test in epidemic area
Early test of fever clinic and CDC
Screening of primary medical institutions
Inspection of floating population in public areas
Performance Characterisitics
Sample tyep: nasal swab Time to result: 15-20 minutes
Sensitivity: 97.70% Specificity: 99.84% Accuracy:98.97%
Layperson study statistics
99.84% of non-professionals carried out the test without requiring assistance
99.82% of different types of results were interpreted correctly.

Parameters:
A Short Test Time: 15 mintues
B Accuracy: More than 98.1%
C Sensitivity: 96.4% Specificity: 100%
D Easy operation: Nasal Swab
E One Step solution
F Lower cost with high efficiency
G Package: 1 Test/Kit, 5 Tests/Kit, 25 Tests/Kit
H It can detection for Omicron Virus

Price and Minumum Quantity
Price FOB in USD: 1.5Minimum Order Qty: 500000 Pieces
Production Capacity: 100 Million Boxes Per WeekPackaging: 1,5,20,25,40,100,200 Pcs Per Box
Sample: Available. Sample Cost Born By Buyer
Product Model And Origin
Brand: jwfModel: jwf01
Seller Code: Jwf
Standards: Ce
Competitive Advantages
Lower cost with high efficiency
Detection for omicron virus
Easy operation
Short test time
Other Details
Omicron covid test kit
Covid test kit
Omicron test card
Covid test card

RT PCR-Detection Kit

Offering Alltest Covid 19 antigen + A+B flue, 1:3 combo tests, single packed, self testing, 2 years self time from my warehouse in Vilnius, Lithuania. Good price from authorised Alltest distributor.

Offering Safecare3 Covid 19 antigen + A+B flue, 1:3 combo tests, single packed, self testing, 2 years self time from my warehouse in Vilnius, Lithuania.

Covid -19 Test Kit (Colloidal Gold Method)
For Self-testing Use (Nasal Swab)
Top 10 In China Having Ce Mark For Rapid Covid - 19 Antigen Self-test


Precise Rapid Sensitive
For At-home Testing
For Early Detection
Able To Sell Across All Eu Member States