Cap: silicone nipple Process: injection blow molding Certification: ISO 9000 Color: white, orange or customized Property: non-toxic, light, good appearance Packaging: sealed in PE bags and put into one carton. Delivery lead time: 7-15 days after receiving the payment. MOQ: 5,000 pieces. Terms of payment: T/T, Western Union, PayPal, L/C Specifications 1.Air-tight food grade borosilicate glass storage jar, keep dry food fresh longer 2.Colorfil lid, food grade and healthy Production information: Pyrex clear wholesale round hot-selling high quality dried fruits storage containers 1.Air-tight food grade borosilicate glass storage jar, keep dry food fresh longer 2.Dishwasher and micro-wave safe glass body 3.Colorful plastic lid, food garden, more eco and healthy 4.Ideal for storing candy, cookies, pasta, snacks, coffee and more 5.Reminding: please wash it before the first use and handle with care Advantage: ·The mould would be provided ·We always put the quality as the first consideration ·Our factory have good reputation ·You can rest assured of our quality, because we are the specialized manufacturer of various pill bottle Manufacturers and have rich experience in production ·Printing: silkscreen printing, off-set printing, hot-stamping, paper label/plastic sticker ·Our pill bottle manufacturers in accordance with the standard of European Union environmental protection. Our quality: 1.With virgin raw material in food grade. 2.Produced in clean workshop. 3.Good match between the cap and the bottle. 4.Neat surface with uniform color and fine gloss. 5.Good shape with smooth edge. 6.EU standard approved. Company information: We are leading manufacturer focus on plastic industry in high-class grade quality with reasonable price. We provide professional plastic products under our brand ?SX?, including plastic reagent bottle, plastic dropper bottle, plastic spray bottle, plastic pill, pharmacy bottle, plastic vaccine bottle, twin (double) neck bottle and more, if any requirements just feel free to contact us. Explanation: 1.The process of plastic oral bottle is extrusion blow molding. 2. Non-toxic, light, good appearance 3. Various colors are available. 4. Better price of plastic oral bottle for large order 5. Logo and words can be printed on the oral-bottle as your requirement. 6. We can produce models according to your own design. 7. We have gained the certification of ISO 9000.
Product description, Product introduction: Calcined petroleum coke is Black or black granular powder, this product has low hardness, high porosity, high carbon content, uniform and stable particle size. Calcined petroleum coke can Improve the performance of friction materials and stable friction coefficient,and reduce thermal recession of friction materials at high temperature. The calcined petroleum coke can be used in disc brake pads and drum brake pads. The character of Fluorite: The petroleum coke is a kind Gum body of strong coking capacity, high carbon content, and can generate high thermal stability when heating. The calcined petroleum coke in the industrial application of the steel and other metal smelting, casting, can be used as gasification and chemical industrial raw materials. The petroleum coke has characteristics of low hardness, light weight, good granularity, and high porosity. When used in friction materials, calcined petroleum coke can enhance the permeability of the friction material, reduce the production of crack and bubble phenomenon. when used in car brakes calcined petroleum coke is not easy to produce noise and stable friction coefficient, reduce friction materials thermal recession at high temperature. It do need uniform and stable particle size which of 40 to 60 mesh of calcined petroleum coke so that to be evenly dispersed in the mixture. Calcined petroleum coke is ideal for friction material regulator, it will be in the friction material will be widely used. property: Ingredients: Si ,A1, Fe ,Mg, S, Na Contains elements can amount to ten kinds, such as sulfide, carbonate, sulfate, silicon oxide ore etc..And the carbon content can be 98% or higher. Grade: 20 to 40 mesh, 40 to 60 mesh, or as per customer requirement
Product Name : Sodium Dodecyl Benzene Sulfonate/Sodium Linear Alkyl Benzene Sulphonic Acid Product Description : 1. High-efficiency emulsifying agent for the polymerization of pressure sensitive adhersive, use level: 1%-0.1%. 2. As raw material for high-grade cleaning agent, eradicator and detergents 3. As anti-static additive for textile oils 4. As excellent anti-static agent for polyester base materials Price of product ( USD price or FOB price) : USD 1250- USD 1560 per MT Product origin : China Key Specifications/Special Features : Active matter % 60±2 70±2 80±2 87±2 Water % : 5 Apparent density kg/cm3 180 Appearance: White to light yellow PH Value : 8 - 11 Place of Origin : China Port Of Loading Shanghai Port,China Payment Term T/T,LC,D/P Delivery Time Shipment within 10 working days" Minimum Order Size and Packaging details : Packing 12.5Kg Bag , Minimum Order Quantity 20 feet container
Product Description : "A large number of Linear AlkylbenzeneSulphonic Acid for the production of a variety of detergents and emulsifiers, such as daily-use chemical detergent, utensils detergents, the cleaning agent of textile industry, dye , electroplating industry, leather industry, degreasing agents, paper industry's decoloring agent." Price of product ( USD price or FOB price) : USD1100~1300 per MT Product origin : China Key Specifications/Special Features : Appearance : Brown Sticky Liquid Active Matter : 96% Free Oil : 2% Inorganic Acid : 1.5% Color,Klett(5% Am aq.sol) : 50" Minimum Order Size and Packaging details : Tanker : 215kg per plastic drum 1050 kg per IBC
Product Description : SLES is an excellent anionic surfactants, soluble in water, with properties of excellent detergency, emulsification, wetting, solubilizing and foaming, anti-hard water performance, and good biodegradability. Widely used in food wash, shampoo, shower gel, soap and other cleaning compound cosmetic products, and construction, textiles, petropleum, leather additives. Price of product ( USD price or FOB price) : USD770~USD830/MT Product origin : China Key Specifications/Special Features : Appearance (25 ): White Viscous Paste Active Matter % 70 ±2 Unsulphated Matter % 3.5 Max. Sodium Sulphate 1.5 Max. 1,4 Dioxane ppm 50 Max. pH(1% ap.sol.) 7.0-9.5 Color(5% am.aq.sol) Klett 30 Max." Minimum Order Size and Packaging details : 170kg per plastic drum,220kg per plastic drum,
Product Description : PRODUCT APPLICATION 1. As a buffer, neutralizer and dough improver, it can be used in pastry and pasta, and it can be used in an appropriate amount according to production needs. 2. As a detergent for wool rinsing, bath salts and pharmaceuticals, alkaline agents in tanning. 3. Used in the food industry as a neutralizing agent and a leavening agent, such as amino acid, soy sauce and pasta such as steamed bread and bread. It can also be added to the pasta with alkaline water to increase elasticity and ductility. Sodium carbonate can also be used to produce MSG 4. Used in the pharmaceutical industry as an antacid and osmotic laxative. 5. The metallurgical industry is used as a smelting flux, flotation agent for ore dressing, steelmaking and smelting as a desulfurizer. 6. The printing and dyeing industry is used as a softener." Price of product ( USD price or FOB price) : USD240~USD260/MT Product origin : China Minimum Order Size and Packaging details : 25KGS bag
Product Description : 1. In Metallurgy industry: as precipitating and separating function for Rare-earth metal. 2. In Dyeing and printing industry: as reducing agent for dyeing and printing industry, as bleaching agent for textile, substitute for Acetic acid, as coloring mordant for fast pigment dyestuff. 3. In organic synthetic: used in 2133 resin, Urea-formaldehyde molding powder, Butadiene catalyst, etc. 4. In electron industry: preparation of chemicals raw material for porcelain capacitor, preparation of electron equipment detergent. 5.In Light industry: polishing for Marble, rust-remover, bleaching, dirt-remover, leather-remover. bleaching. 6. In other aspects: as analysis reagent, raw material of chemicals." Price of product ( USD price or FOB price) : USD 460 ~ USD 490/MT Product origin : China
Dust-free antimony trioxide is made from fine Sb2O3 powder treated by special techniques to moisturize it slightly thus avoiding damages to human body that may be caused by dusts in the application of the product while achieving high fire-retarding efficiency and plasticization. It is easier to combine with base materials and even dispersing. This product is applied mainly as retardant additive and plasticization for polyvinyl chloride, synthetic fiber, synthetic rubber and vinyl resin, etc. Upon customerâ??s request, we can adopt diversified ingredients of organic solvents such as mineral oil, Chlorinated Paraffin, glycol, DOP, DIDP, etc.
Monofilament polyester yarn, dope dyed with masterbaches, round, bright. Characteristics: eco-friendly, high color fastness, consistent color, comfortable luster, soft touch, many color alternatives etc. Our products are awarded with oeko-tex standard 100, iso9001:2000 and iso14001 certificates and "the certificate of green fiber" issued by the china chemical fiber association. Applications: textile fabrics, embroidery, label, decoration, braid, jacquard, knitting, home textile, clothes, shoes, hat, carpet, curtain, scarf, bag, towel, gloves, socks, webbing, tie, wedding dress, curtain, cushion, printing mesh, filter, fishing tools, sewing thread, wool blended fabrics etc.Etc -
When the epidemic is waiting for an opportunity again, to quickly control the spread of the virus, nucleic acid testing has become the only way to go, and it has also attracted attention from all walks of life. However, there are many factors that affect the results of nucleic acid testing, including sample collection location, collection method, storage and transportation process, nucleic acid extraction operation, and so on. Among them, the stability of the sample is a key link. How to quickly inactivate the virus and protect the viral nucleic acid from being degraded, maintaining the stability of the virus sample storage solution has become a top priority. Step 1: Break the shell and eliminate the "combat power" Like other RNA viruses, the results of the new coronavirus are simply composed of a protein shell wrapped in single-stranded nucleic acid-RNA. We can simply think of the RNA of the virus as the "brain" that gives orders, and the protein is the "limbs" that execute orders. The new coronavirus infects the human body and reproduces and causes symptoms. This process is mainly completed by the biologically active protein structure outside the virus. The inactivated virus preservation solution is mainly the virus modified by the nucleic acid extraction lysis solution; it is a powerful protein denaturant that can quickly dissolve the protein and cause the virus structure to be broken. When the clinical sample with the new coronavirus is mixed with the preservation solution, the virus in the sample is affected by the lysis solution, and the protein shell is quickly destroyed, and this damage is irreversible. At this time, the virus has lost its evil "minions" and no longer has the ability to infect people, thus ensuring the safety of contacts during sample transportation and processing. Step 2: Inhibit enzyme activity and escort the "ID card" After the shell is lysed, the RNA of the new coronavirus is free in the preservation solution. As a single-stranded macromolecule, the stability of RNA is not as immobile as double-stranded DNA. The RNase that exists everywhere in the natural environment is the culprit that causes RNA to hydrolyze and break. Therefore, it is generally considered that the viral RNA that has lost its outer shell protection is In addition to the inactivated virus storage solution, the virus preservation solution produced by Desheng also has a non-inactivated virus storage solution. It retains the protein coat of the virus and the viral nucleic acid DNA or RNA at the same time, so that the virus has the integrity of the protein epitope and nucleic acid in vitro. Of course, there is a certain risk of infectivity when operating errors. Long-term storage after sampling needs to keep strictly low temperature. Regardless of the virus preservation solution, we need to strictly operate, store or transport in accordance with the requirements when using it.
1. Tris brief introduction As the handling in biological buffers, tris plays a more important role, not only because of the wide pH range of tris, it can act as a pH regulator. Conventionally, it is white crystal particles, but there are certain requirements for water solubility, and white powder will not meet its requirements better. The pH value of the acid-base indicator is 7-9, and there are other names, tromethamine, ammonium tromethamine, trishydroxymethylaminomethane and so on. Although they are all the same product, different buyers will call them differently based on different fields. Second, the use of Tris 2. Tris application in cosmetics With the improvement of peoples quality of life, more and more attention is paid to spiritual life. Among them, the heart of beauty is common to everyone, regardless of men and women: appearance has become an important indicator of first impression (of course I strongly disagree Because I dont have that beauty. The rise and mass use of cosmetics have become inevitable. To make good cosmetics, the ingredients are very important. The role of Tris in cosmetics is basically to adjust the pH, and it can also be used as a neutralizer. The PH value of healthy Asian skin is slightly acidic, and the ingredients in many cosmetics are very complicated. If you want to adjust the pH value, you must use tris to adjust, so that the entire raw material composition will not change too much. Many people see that tromethamine is a chemical product, and it is very scary to use in cosmetics, and they are afraid of being unsafe. In fact, if the proportion of ingredients is used properly, there is no need to worry about it. Generally, it will not harm the skin. 3.Tris is used for synthesis Tris with a relatively simple structure can synthesize many products, and the same buffer system, Tris-HCL, Bis-Tris Tricine, TES, TAPS, etc. can all be synthesized with tris. Compared with others, the price of Tris will be cheaper, but the synthesized products can be applied to more fields, and the price will be doubled. Therefore, the synthesis of tris can be said to be a hot pastry.
Applications - UV nail polish - UV varnish or UV inks for paper - UV undercoat for furniture - UV coatings for PVC/wood floor *** Principal Properties - Curing fast, curing rate is close to the stander epoxy acrylate - The firm with toughness and hardness after curing - Cost-effective - Yellowing resistance is not require
Applications - UV wood coatings - UV varnish *** Principal Properties - Fast curing - Good wettability - Low yellowing - Good toughness and flexibility - Good compatibility with other resin
1 ml , 3 ml safety syringe
The inactivated preservation solution is a kind of virus preservation solution, which is mainly used to store the virus after the inactivation of the virus, and the inactivated virus preservation solution can effectively prevent the second infection of the user. Its characteristic is that it can quickly inactivate the virus, degrade the viral protein membrane capsid, and release the viral nucleic acid. At present, the new coronavirus that is sweeping the world in society is to release and degrade RNA viruses, so that post-reverse transcription PCR amplification experiments can be performed to achieve the purpose of using nucleic acid to detect viruses. In addition to the lysing components, the inactivated virus preservation solution usually contains detergents that denature proteins and destroy the membrane structure, biological buffers, inhibitors that prevent nucleases from degrading nucleic acids, and reagents that maintain the stability of the nucleic acid structure. The lysis system may also add protease to cut the protein into small fragments to promote the separation of protein and nucleic acid. At present, there are many known methods for lysing viruses. Two of the more common methods are heating method and concentrated salt method: The concentrated salt method uses high concentration of salt to destroy the secondary bond between nucleic acid and protein, disassociating the nucleoprotein and releasing the viral nucleic acid. Usually, guanidine hydrochloride, guanidine isothiocyanate or non-guanidine salt cleavage salt can be used to directly lyse and inactivate the virus without heating the sample, which is relatively simple. The heating method is based on heating to a temperature value of 80 C ~ 100 C, because generally samples are frozen at -70 C, generally need to be heated for 5 to 10 minutes, this operation is relatively simple, only one step can be used for The template for nucleic acid PCR amplification does not require any operation steps such as centrifugation and extraction, and is faster than the commonly used CheleX100 extraction method and phenol/chloroform methods. Although this method is simple, the amount of nucleic acid extracted will be relatively small. Since the outbreak of the epidemic, Hubei Xindesheng Materials Co., Ltd. has been devoted to the research and development of virus preservation solutions, striving to provide society with its own strength. After many experiments and tests, Desheng has been able to ensure that the product quality and experience are very up to standard. Among them, our company produces inactivated and non-inactivated virus preservation solutions, which can be adjusted and selected on demand. In addition, Desheng reminds our customers that when purchasing virus preservation solutions, you should not blindly pursue low prices. You can take samples separately and go back for testing and comparison. The best is the best.
With the recurrence of asymptomatic, nucleic acid testing cannot be stopped for a moment. This is not only a protection for everyone, but also a protection for yourself. Speaking of nucleic acid testing, we have to bring up our virus sampling tube again. Its choice also plays a key role. Why use a virus sampling tube? Virus detection is different from conventional biochemical detection. The virus itself is a simple microorganism that must be parasitic in living cells. After sampling, the virus leaves the host cell, and its protein shell and nucleic acid will be quickly degraded in the sampling tube, so the nucleic acid During the test, it is impossible to determine whether the initially collected sample contains the virus, and it is easy to cause false negatives. What are the requirements for an excellent virus sampling tube? 1. In terms of sample effectiveness: The non-inactivated virus preservation solution must maintain the activity of the pathogen's infectious agent. Preferably, it can preserve the activity of the virus at room temperature. The inactivated virus preservation solution needs to inactivate the virus but maintain the nucleic acid of the virus to meet the time from sample sampling to laboratory testing. It is necessary to limit and prevent the reproduction of symbiotic microorganisms to ensure the reliability of diagnostic tests. 2. In terms of safety: Because the virus sampling tubes are basically all infectious substances, and some are highly pathogenic infectious substances, the requirements for packaging containers are very strict, and they need to meet safety and ensure that liquids do not leak during transportation. . So what is the virus preservation solution? Under what circumstances do I need to use a virus preservation solution? The virus preservation solution is a protective liquid medium added to the virus sampling tube to protect the sample after the nasopharyngeal swab is sampled. Normally, nucleic acid PCR cannot be directly performed at the sample collection site during nucleic acid detection. If the sample collected by the swab needs to be transferred and transported, it is necessary to add a virus preservation solution. Why is the virus preservation solution divided into inactivated and non-inactivated? After the virus samples are collected, there is usually no way to test in time at the sample sampling site, so the collected virus swab samples need to be transported, and the virus itself will be quickly lysed outside the body and affect subsequent testing, so when storing and transporting , You need to add a virus preservation solution. For different detection purposes, you need to use different virus preservation solutions and different virus detection experimental conditions, so it is divided into two types of preservation solutions, inactivated and non-inactivated. There are no other microorganisms, causing the virus to decompose after sampling or other influences causing false detections.
Every biological system has a well-known buffer system to maintain the acid-base balance within a specific pH range. Our lives depend on the function of the buffer system. A buffer system is a solution that resists changes in pH when acid or alkali is added. Different buffers have different ability and efficacy for skin replacement. The high-quality Good's Buffers produced by Desheng are usually used in cosmetics, such as toners, essences, lotions, eye creams and facial masks, etc., according to your application. Here are 4 biological buffers commonly used in cosmetics. 1) HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) CAS Number: 7365-45-9 Useful pH range: 6.8-8.2 The main purpose 1. Acidity regulator, or pH control agent. 2. An effective concentration of HEPES can provide an exfoliating effect. 3. Promote the transdermal absorption of various functional ingredients in cosmetics. 2) TRIS (Tris(hydroxymethylaminomethane)) CAS number: 77-86-1 Useful pH range: 7.2-9.0 use: 1. Non-ionic surfactants-amines 2. Acidity regulator or pH control agent. 3. Excellent cleaning ability and emulsifying ability. 3) BIS-TRIS (Trimethylol) Methylamino Propane) CAS Number: 5625-37-6 Useful pH range: 6.1-7.5 The main purpose 1. Non-ionic surfactants-amines 2. Acidity regulator, or pH control agent. 3. Excellent decontamination, dispersion, emulsification and antistatic ability. 4) Glycylglycine buffer (G-132) CAS Number: 7365-44-8 Useful pH range: 7.5-8.9 The main purpose: 1. Adjust the pH range of the solution 2. Condition the skin, reduce pores and prevent skin aging. 3. Excellent emulsification, dispersion, antistatic, wetting, penetration, and decontamination capabilities Precautions External factors, water and detergents may reduce the local buffering capacity, because the elution of buffer chemicals leads to increased pH and irritant contact dermatitis. Inflammatory diseases, including atopic dermatitis, psoriasis, and acne vulgaris, increase in pH and decrease buffering capacity. For the treatment of skin diseases and aging skin, emollients with a pH value slightly higher than the average normal pH value and with appropriate buffering capacity should be used first. Desheng is a professional manufacturer of biological buffers. There are many cooperative manufacturers in the skin care industry. In addition to the two biological buffers Hepes and Tris, it also produces many other models such as Mops, Taps, Pipes, Caps, and the core product tris buffer. Known for its ability to maintain the original color of emulsions and excellent odor suppression capabilities, it is widely used in cosmetics such as skin care lotions, sunscreens, eye makeup products, nail polish, etc. It can maintain the functional effectiveness of active substances and is a skin care product The industry's trusted choice.
Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is usually performed in higher vertebrates, and its reference value has been established, but fish hematology still needs further research. Many internal and environmental factors have a profound impact on the hematology value of fish, making it difficult to determine the reference value. In addition, due to the short clotting time, fish blood usually requires the addition of anticoagulants. The choice of anticoagulant is essential to obtain reliable blood test values. In this study, the effects of two common anticoagulants, K 2 EDTA (1.8 mg/ml) and lithium heparin (18 IU/ml), the hematological value of brown trout in the breeding season on the spawning season The spawner conducted an investigation. The results of basic hematology analysis, such as compacted cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and White blood cell count (WBC) was compared between K 2 EDTA and lithium heparin. Statistically significant differences were observed in PCV, MCV, and MCHC, while HGB, RBC, MCH, and WBC did not show such differences. These results indicate that lithium heparin provides more reliable results because the red blood cells in the K 2 EDTA-treated samples have a tendency to swell. The salt of ethylenediaminetetraacetic acid (EDTA) is recommended by the International Committee for Standardization in Hematology (ICSH) and the Clinical and Laboratory Standards Institute (CLSI) as the anticoagulant of choice for blood counts. EDTA acts as a chelating agent for Ca 2+ and Mg 2+ ions. It binds to calcium and is essential for the enzymatic reaction in the coagulation cascade, resulting in anticoagulation with minimal impact on cell morphology. EDTA can most reliably preserve the cells on the stained blood membrane. It is commonly used for routine hematology tests in humans, mammals and other vertebrates. It has also achieved some success in fish. However, EDTA salt has some disadvantages. In some cases, they can cause blood hemolysis, especially in specimens anesthetized with unbuffered tricaine mesylate (MS 222). Lithium heparin is more suitable for hematological evaluation of spawning in trout than dipotassium ethylenediaminetetraacetate. Heparin does not affect the size of red blood cells, thereby minimizing the possibility of hemolysis. Compared with heparin, blood smears made from blood treated with EDTA are of higher quality. Based on current research, we suggest that K2EDTA can be used cautiously for blood count and HGB determination. In order to better understand the effects of anticoagulants on hematological parameters, further research is needed.
Tris, or tris(hydroxymethyl)aminomethane, has been widely used as a pH buffer in biological media for about 35 years. The near-ideal properties of this biological buffer are the reason for its popularity. Tris does not absorb water, is easily soluble in water, and has high purity. It does not precipitate calcium salts, is stable in solution for several months at room temperature, and does not seem to inhibit many enzyme systems. However, due to the chemical and physical properties of Tris buffer, improper use may lead to erroneous pH measurement of the electrode system. The influence of pH in TRIS buffer on electrode Biochemists use TRIS buffers to control the pH within the physiological range (approximately 7 to 8 pH), because phosphate can cause adverse reactions with biological substances in the test sample. However, when pH measurements are to be made on these solutions, another type of "adverse side reaction" must be recognized, involving the pH electrode system. The influence of chemical and physical properties of Tris buffer on pH Tris, which is commonly used for physiological measurement (pH 7 to 7.5), does not have a large buffer capacity. Therefore, caution should be exercised when using tris buffer to control sample pH. The rather large temperature coefficient of tris (-0.028pH/ C) is also worth considering. For example, if the meter is calibrated with tris buffer and the tris temperature is 20 C, using a pH value of 25 C will cause errors. Therefore, temperature pH dependence must be used when using tris for standardization. When using tris to control the pH of the sample, the degree of control will depend on the sample temperature. If the sample temperature changes by 2 C, the pH control changes by about 0.06 pH units. The required pH control will determine the maximum allowable temperature fluctuations. Advantages of Tris buffer Because the Tris base is highly alkaline, this buffer system can be used to prepare buffers with a wide range of pH values from acidic to alkaline; It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions. Disadvantages of Tris buffer The pH value of the buffer is greatly affected by the concentration of the solution. The buffer is diluted ten times, and the pH change is greater than 0.1; the Tris buffer should be prepared from high-purity tris and HCl using distilled or deionized water without CO2. The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer, so it must be prepared at the use temperature, and the Tris-HCl buffer prepared at room temperature cannot be used at 0 C to 4 C; It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed; This buffer has a certain interference effect on some pH electrodes, so use an electrode compatible with Tris solution.
In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment. When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers. Prepare TRIS base and TRIS-HCL buffer Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O. Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L. Prepare HEPES buffer To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C. Prepare PIPES buffer To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O. Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.
