What is PIPES buffer PIPES Buffer (CAS5625-37-6) chemical name is 1,4-piperazine diethanesulfonic acid, which is a zwitterionic biological buffer. It is a white powder solid at room temperature. PIPES Buffer is not easily soluble in water (1g/L (100C). It can be dissolved by mixing equimolar sodium salt solution and titrating to the appropriate pH value. For example, PIPES Buffer (CAS76836-02-7) and PIPES sesquisodium salt ( CAS100037-69-2). Application of PIPES Buffer PIPES Buffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscopy research. PIPES Buffer is similar to MES Buffer and MOPS Buffer (also produced by Desheng). This Good's Buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non-coordinating buffer in metal ion solutions. Advantages of PIPES Buffer According to related literature, the use of NaOH-PIPES buffered glutaraldehyde solution in the ultrastructure of various tissues, such as human skin, human hypertrophic scars, mollusk neuron tissue, etc., the results show that more organelles and organelles can be observed Between the details. The cytoplasm is more uniform in consistency. Compared with NaOH-PIPES buffer, microfilaments and microtubules are more common in PIPES tissues. In addition, PIPES Buffer is proven to be comparable in living cells. What is TRICINE buffer TRICINE Buffer (CAS 5704-04-1), a zwitterionic biological buffer containing amine and acid groups. This product is a white crystalline powder, slightly soluble in water (the solubility is 1.8g/100ml water). Application of TRICINE Buffer TRICINE is widely used in cell culture and electrophoresis research. As a buffer for cell culture research, TRICINE can inhibit the unexpected growth of mycoplasma in animal tissue culture. It has also been used in bacterial culture to prevent precipitation of iron salts. The Good's buffer can form complexes with most metal ions, such as Mg, Ca, Co, Cu, Ni, and Zn, so the stability constant and concentration should be considered when choosing the buffer. As a buffer for electrophoresis research, Tricine has been used as an alternative to protocols using the controlled substance barbital. Advantages of TRICINE Buffer TRICINE has a higher negative (more negative) charge than glycine, making it migrate faster. In addition, its high ionic strength results in more ion movement and less protein movement, so that low-molecular-weight proteins can be separated in a lower percentage of acrylamide gels. Tricine has been proven to be used to separate proteins in the range of 1 to 100 kDa by electrophoresis. In ten tests using firefly luciferase for ATP determination, 25mmol/L tricine buffer was found to be the most effective buffer.
Luminol can be oxidized by many substances to produce chemiluminescence, which is the earliest research and most widely used luminescence reagent. The chemiluminescence analysis of metal ions occupies an important position in the entire research and application of chemiluminescence analysis. Some metal ions can enhance the chemiluminescence of the luminol system; some ions can inhibit the chemiluminescence of the luminol system; and some ions can directly oxidize the luminol to produce chemiluminescence. In our drug analysis, we use the catalysis or inhibition of metal ions and compounds for direct or indirect detection. Let's take a look at how luminol "extraordinarily" in drug analysis. Application of Luminol Luminescence System in Pharmaceutical Analysis 1. Direct or indirect detection using the catalysis or inhibition of metal ions and metal ion compounds, such as the determination of sodium nitroprusside (SNP) and catechol compounds. Certain reducing substances such as citric acid, citrate esters, Vc, ascorbic acid, etc. can also be detected by the metal ion-luminol luminescence system. 2. The enhancement or inhibition of the chemiluminescence reaction of luminol by biomolecules and organic substances. DNA, albumin, glucose, and uric acid can enhance the chemiluminescence reaction of luminol, thus establishing their own detection methods. In an alkaline medium, the inhibitory effect of ascorbic acid on the Luminol-K3Fe(CN)6 system can be used to determine ascorbic acid. Luminol-K3Fe(CN)6 series can also be used to monitor glucose and lactic acid. Thiol-containing drugs, promazine, etc. have an inhibitory effect on the luminol luminescence system, and a detection method is proposed accordingly. 3. Preparation of expendable chemiluminescence sensor. Luminol and other reagents are fixed on ion exchange resin to develop a chemiluminescence flow injection sensor, which is used for the determination of Vc. Combine enzyme immobilized column with FIA, use immobilized enzyme reactor, detect by luminol, it has been used for glucose, alcohol, oxalate, lactate, choline, uric acid in serum and urine, etc. Detection. Seeing more of the power of luminol, we suddenly discovered that it is acceptable to us wherever it is. This also fully illustrates its value. If you want to know more about luminol's knowledge and uses, you might as well find its manufacturer-Desheng. I believe you will be worthy of asking.
AirMMax roots blower is three lobe type roots blower with pressure reaching up to 150kpa in single stage roots blower and pressure reaching up to 200kpa in double stage roots blower, air capacity reach up to 30000m3/hour, which cover most water treatment plant, sewage treatment plant and effluent treatment plant in industrial and municipal wastewater treatment plant. Roots blower inculds: blower body motor , base , inlet sliencer, outlet sliencer, safety valve, pressure gauge, check valve, flexible joint, belt cover, bolts&nuts
The new Trinder's reagent is a highly water-soluble aniline derivative that has been widely used in diagnostic tests and biochemical tests. In the colorimetric determination of hydrogen peroxide activity, it has many advantages compared with conventional color reagents. The new Trinder's reagents are stable enough to be used in solutions and test line inspection systems. Currently chromogen substrates (new Trinder's reagents) include TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, etc. These new chromogens actually have similar reaction principles to traditional chromogens, but they have more advantages. We mainly understand the MAOS among them. The full name of MAOS is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt. It is a highly water-soluble aniline derivative of the new Trinder's reagent. Which has a pivotal position. It is a water-soluble reagent that can be applied to the enzymatic photometric determination of hydrogen peroxide, and is widely used in diagnostic testing and biochemical tests. Reasons for the discoloration of the reagent solution: Color-developing substrates such as MAOS are all new Trinder's reagents, which are derivatives of highly water-soluble aniline sodium salt, which will produce red or yellow substances after being oxidized. Trinder's reagent powder is not easy to be oxidized. It needs to be sealed and refrigerated after preparation. If the solution is exposed to the air for a long time and the temperature is high, they may be slowly oxidized by the oxygen in the air. Discoloration of the solution occurred. MAOS is sensitive to light and sensitive to humidity. Therefore, when we store, the storage temperature of cold storage is between 0-10, and the temperature of frozen storage is below 0. If the storage temperature is not specified, in principle, it should be stored in a cool place below 15C. Hubei Xindesheng has focused on the research and development of a batch of new Trinder's reagents and other color reagents. The new reagent has more advantages: such as high water solubility, UV absorption of the color reaction product> 540nm; color reaction requires a wide pH range, and the activity of a variety of enzymes can be guaranteed during the reaction; color reaction sensitivity is higher and can be used For the determination of very small amounts of analytes. And it has considerable product advantages, purity: >99%, good water solubility, stable process, and can ensure the appearance of the product is pure white crystal powder. Welcome everyone to inquire.
4-Hydroxyethylpiperazine Ethane Sulfonic Acid (HEPES) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research. As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products: 1. Advantages of PH range: The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture. Purity advantage: Desheng HEPES has a purity of >=99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services. Reserve advantages: Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment. Export advantages: Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention. HEPES packaging advantages: Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.
Hubei Xindesheng was established in 2017. It is a new technology-based enterprise established on the basis of Wuhan Desheng Biochemical Technology Co., Ltd. (2005). After years of innovation and development, the company has now become a leader in "blood collection tube additives", "chemiluminescence reagents", "biological buffers", "color reagents", etc., integrating R&D, production, sales and service. high-tech enterprises. Tris, as the company's main advantage product in biological buffers, has always had its own advantages: 1. TRIS is used as a neutralizer in cosmetics, which is actually a PH regulator. 2. Tris is used as a solvent for nucleic acids and proteins and as a biological buffer. 3. It can be used to prevent and treat metabolic acidosis and respiratory acidosis; 4. Tris is used to prepare surfactants, vulcanization accelerators and intermediates for some drugs. 5. Tris mainly acts as a pH regulator, cross-linking accelerator, polyester coating raw material and phase change core material in the coating preparation process; 6. Products related to Tris can be prepared through the molecular structure of Tris. Such as Bis-Tris, Tricine, TES, TAPS, etc. From the above points, we can see that Tris itself has a wide range of applications, but it is not a simple matter to make high-quality Tris. In the past ten years, Desheng has been learning and improving its technology. The Tris produced is white crystalline powder; it has good solubility in water systems and minimal solubility in organic solvents; it is in dry or solution state. It has excellent stability and can resist enzymatic or non-enzymatic degradation; it has a very small absorption value for visible light and ultraviolet light. Therefore, when we store tris, we only need to store it in a cool, dry and moisture-proof warehouse at room temperature. Desheng is a professional TRIS manufacturer, committed to providing customers with high-quality products and services that can meet the special requirements of R&D customers for product types, packaging, purity and other aspects.
Luminol has become one of the most widely used chemiluminescent reagents due to its advantages of high quantum yield, easy synthesis, and good water solubility. Its development process is the same as the arduous process of human beings. From the beginning, there is not much understanding, to the current glow and fever, it can only be summarized in one sentence: gold always shines. In the future, there may be more uses we don't know to benefit our lives. We only need to learn more about it to discover its unlimited potential as soon as possible. Application in the analysis of inorganic substances Using inorganic ions to enhance or inhibit the chemiluminescence of the luminol system, these metal ions can be measured. It is worth pointing out that some metal ions have higher catalytic activity than metal ions after being combined with complexing agents. For example, using the luminol-H2O2-silver phenanthroline system, the detection limit of silver ions can reach 2.7 10-g/L3. The use of sensitizers can improve the sensitivity of detection. The sensitization effect of triphenylmethane derivatives on the luminol luminescence system. It was found that Coomassie Brilliant Blue G250 had the best sensitization effect on the luminol-H2O2-Cu2+ system. A reverse flow injection technique was used to establish a measurement of Cu2+. The new method has a detection limit of 15. The use of the local concentration effect of surfactants to achieve micelle sensitization has been increasingly used in luminescence analysis. For example, the reversed micellar system FIA method is used to measure V4+, the detection limit is Luminol chemiluminescence analysis has been widely used in various fields, and new methods and new technologies are constantly emerging, especially the combination with FIA, HPLC and other technologies, which overcomes the shortcomings of poor selectivity in luminescence analysis and makes the analysis more flexible. Emblem and accuracy. Because of Rumi's stable performance and low price, practical application research and analysis will have broader application prospects.
Food safety issues have not only seriously affected the health of the people's diet, but also hindered the export trade of my country's agricultural products and food, and undermined the international reputation of my country's food industry. Only by strengthening basic food safety research, especially rapid testing research, can we adapt to the development under the new international situation. How to quickly and effectively do a good job of testing, we have to bring up our powerful chemiluminescence immunoassay to see through which aspects it plays a role in food safety testing. Detection of microorganisms in food The inspection of microorganisms in food is mainly to inspect general pollution and pathogenic bacteria. Escherichia coli O157 H7 is an important food pathogen. The enhanced luminol peroxidase luminescence system can quickly detect E. coli O157:H7. The method is simple and fast. The minimum detection concentration of each bacteria is in the range of 104105CFU/L, and the recovery rate is between 90% and 120%. Detection of biotoxins in food Biotoxin is a general term for a large class of biologically active substances, including animal toxins, plant toxins and microbial toxins. Biotoxins have posed a serious threat to food safety and human health. For example, the chemiluminescence enzyme immunoassay method for detecting aflatoxin M1 in milk uses horseradish peroxidase to catalyze luminol to emit light. Detection of pesticide residues in food Pesticide residues in food can cause chemical food poisoning, and low-concentration residues are also potentially harmful to the human body. At present, the most researched pesticide residue detection technologies are mainly in vivo detection methods, chemical detection methods, enzyme inhibition methods and instrumental analysis methods. modified products After recognizing the major changes brought to us by chemiluminescence reagents, Desheng has independently developed and produced chemiluminescence reagents since 10 years ago, including luminol, isoluminol, and acridinium ester and acridinium ester derivatives. product. These products are not only recognized by many companies in China, but are also well received even if they are exported overseas. Low-key life, high-profile work. What's more, a good product in the eyes of the public always speaks with strength.
Carbomer is a commonly used rheological additive. It has excellent thickening, suspending and stabilizing abilities. Different products have long and short rheological properties. The appropriate product can be selected according to the formula. Carbomer can improve the sensory properties of cosmetics. For example, adding carbomer gel or lotion formula will quickly decompose on the skin and other salt-containing substrates. This rapid decomposition will produce high moisturizing feeling, light spreading feeling and refreshing sense. It is the finishing touch in our cosmetics. For cosmetics, its senses are very important. After all, cosmetics are used on parts of our body. Good sense organs give people a pleasant experience, and bad sense organs may make us lose confidence in a certain product. Carbomer plays a big role in cosmetics. Advantages of Carbomer's finishing touch: It does not irritate human skin and eyes. After a lot of toxicological studies, it is found that it is safer than salt; The shampoo has high-efficiency and long-lasting suspension stability. The acrylic resin cross-linked polymer can improve the suspension of silicone oil. Compared with cellulose and guar gum, it has higher suspension stability; Improve foam stability in low foam system; It has salt resistance. The acrylic resin cross-linked polymer can still maintain a higher viscosity in a system with salt, and is less affected by the pH value of the solution or electrolyte concentration, and has a wider range of applications; Easy to use, short wetting time, saving a lot of manpower, material resources and time; Good antiseptic performance, not easy to be decomposed by microorganisms, and easy to store; It has excellent rheology, can adjust the viscosity of the product, improve the thixotropic properties of the gel system, and improve the stability of the product. With high rheological value, it can permanently suspend insoluble components in a low-concentration system, making the product uniform and stable, while other thickeners such as cellulose cannot provide suspension. Carbomer uses these advantages in cosmetics, let us rush to it. The quality of Cocarbom itself also plays a key role. Good carbomer quality can achieve the desired effect of the product. There are not many domestic manufacturers that really do carbomer. And Desheng is one of the manufacturers that develops and produces carbomers, and has consistently given high evaluations under the use of many companies, whether it is product quality or product service. However, Carbomer 940 and 980 are being developed and produced by Desheng temporarily, and the supply can still meet the needs of customers. You can now apply for a free sample online for trial, please feel free to contact us if you need it.
The structure and function of the protein need to be maintained in a suitable pH environment. Therefore, the choice of pH buffer is very important during the preparation and purification of the protein. Only a suitable buffer can ensure the activity and structural stability of the protein. Effect of buffer in protein purification process There are many kinds of buffers for protein purification. While maintaining the pH of the system, it is necessary to ensure that the protein cannot be inactivated during the purification process, or to minimize the amount of protein inactivation, which must be maintained in every step of protein purification. Solubility and activity of protein. Protein purification buffer requirements The buffer in the protein purification process should not only prevent protein degradation, but also prevent protein aggregation, which has a significant impact on the experimental results. The following factors should be considered when selecting and reagent buffers: pH, buffer system, salt ions, reducing agents and stabilizers. Each of these factors should be optimized according to the purified target protein, and the activity of the target protein should be used as an evaluation criterion for optimization. Understand protein stability conditions There are many types of proteins, and different proteins adapt to different pH values. The buffer should be selected according to its optimal pH value. In many cases, the pH is 7.4, which is a mimic biological condition, and most proteins are stable at this pH. However, there are some exceptions, which require pH adjustment to keep the protein soluble and not degraded. Commonly used buffers for protein purification Commonly used buffers in the protein purification process include Tris, Tricine, Bis-Tris, MOPS, etc. Among them, Tris is commonly used. Its buffer range is close to physiological pH and has a low ionic strength. It is a good solvent for proteins. Can ensure the stability of the protein. It should be noted that when preparing the buffer, try to avoid the isoelectric point pl of the target protein. Because the protein has no electrostatic charge on its surface in the pH solution of its isoelectric point, the repulsive force between the hundred molecules is reduced, it is easy to aggregate, and the solubility is reduced. Now the adaptation pH value, isoelectric point, and buffer range of the buffer to be purified can be found, and you can directly match it according to the corresponding requirements. Desheng is a manufacturer of in vitro diagnostic reagents, which can provide large-package pH buffers such as Tris, MOPS, Bicine, etc.
Chinese Name: morpholine ethanesulfonic acid sodium salt English Name: MES sodium salt CAS No.: 71119-23-8 Molecular formula: C6H12NNaO4S Molecular weight: 217.22 Storage conditions: Store at RT
P110 OCTG casing pipe Company Hebei Borun Petroleum Pipe Manufacturing Co.,Ltd Product Name OCTG casing pipe SurfaceTreatment Anti-corrosion Water Based Paint Standard API SPEC 5CT Steel grade H40, J55, N80, N80Q, L80, L80-1, L80-9Cr, L80-13Cr, P110 etc O.D 114.3~508mm W.T 4.24-16mm Length R1( 6.10-7.32m ), R2( 8.53-9.75m), R3(11.58-12.80m) packing details Protection Caps on both ends and in bundles with steel strips and two tags as per export requirements Type of upset NUE, EUE, Plain end MOQ 10Ton Payment term T/T,L/C Delivery date According to The Specifications And the Quantities Of Each Contract Application Used as the architectural retainer for the walls of oil, gas wells Main Market South and East Asia, Middle East, Europe and America
piperazine-N, N '- di (2-ethanesulfonic acid) |English name|PIPES |Chinese name|Piperazine-N, N '- bis (2-ethylsulfonic acid) / piperazine-1,4-diethylsulfonic acid |CAS#|5625-37-6 |Molecular weight|302.368 |Molecular formula|C8H18N2O6S2 |Storage condition|room temperature, away from light and moisture |Application|This product can not form stable complex with most metal ions, and can be used as buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, PIPES is not suitable for redox system because it can form free radicals. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because pipes has relatively large ionic strength and its pKa value is concentration dependent. |Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder. Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer. Enterprises or individuals in need can directly contact . We look forward to your calls, to join hands in creating a better tomorrow!
piperazine-N, N '- di (2-ethanesulfonic acid) |English name|PIPES |Chinese name|Piperazine-N, N '- bis (2-ethylsulfonic acid) / piperazine-1,4-diethylsulfonic acid |CAS#|5625-37-6 |Molecular weight|302.368 |Molecular formula|C8H18N2O6S2 |Storage condition|room temperature, away from light and moisture |Application|This product can not form stable complex with most metal ions, and can be used as buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, PIPES is not suitable for redox system because it can form free radicals. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because pipes has relatively large ionic strength and its pKa value is concentration dependent. |Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder. Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer. Enterprises or individuals in need can directly contact (Wechat is the same number) . We look forward to your calls, to join hands in creating a better tomorrow!
Chinese Name: disodium 1,4-piperazine diethylsulfonate English Name: Disodium piperazine-1,4-diethanesulphonate CAS No.: 76836-02-7 Molecular formula: C8H16N2Na2O6S2 Molecular weight: 346.33 Storage conditions: Store at RT Application: biological buffer
Chinese Name: piperazine-N, N '- bis (2-hydroxypropylsulfonic acid) sesquisodium salt English Name: Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium salt CAS No.: 108321-08-0 Molecular formula: C20H41N4Na3O16S4 Molecular weight: 790.79
PIPES-Na Chinese Name: piperazine-N, N '- bis (2-hydroxypropane sulfonic acid) disodium salt English Name: Piperazine-N,N'-bis(2-hydroxypropanesulphonic acid) disodium salt CAS No.: 108321-07-9 Molecular formula: C10H20N2Na2O8S2 Molecular weight: 406.38
Chinese Name: piperazine-N, N '- bis (2-ethanesulfonic acid) sesodium salt English Name: PIPES sesquisodium salt CAS No.: 100037-69-2 Molecular formula: C16H33N4Na3O12S4 Molecular weight: 670.68 Storage conditions: Store at RT Application: biological buffer
Chinese Name: bis (2-hydroxyethyl) amino (trihydroxymethyl) methane English Name: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol CAS No.: 6976-37-0 Molecular formula: C8H19NO5 Molecular weight: 209.24 Storage conditions: Store at RT Purpose: It can be used in biological buffer for detecting creatine kinase. It is also used to protect hemoglobin in freeze drying. Cathodic electrolyte is used in IEF/2-D gel electrophoresis.
|English name|N-Cyclohexyl-3-aminopropanesulfonic acid |Chinese name|3-(cyclohexylamine)-1-propanesulfonic acid |Abbreviation|CAPS |CAS#|1135-40-6 |Molecular weight|221.32 |Molecular formula|C9H19NO3S |Storage condition|room temperature, away from light and moisture |Application|Tris Gly, SDS and 20% methanol buffer are not used for PVD membrane transfer of protein. Because there is glycine in the buffer, it is difficult to distinguish whether glycine is brought in by buffer or the protein itself during sequencing. Now, 10mm / 1caps10% methanol is used as membrane transfer buffer for sequencing, and the methanol concentration range in caps electroimprinting buffer is 0-20%. Generally, the methanol concentration is high The buffer has a good effect on the transfer of low molecular weight protein, while the buffer with low methanol concentration or even without methanol is conducive to the transfer of high molecular weight protein. |Product advantage|The purity is |99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
